? Recombineering Used to Engineer the Genome of Phage T7
...Here we find that T7 DNA, introduced by either infection or electroporation, can be genetically modified in vivo by recombineering using ? Red-mediated recombination. We also demonstrate that the efficiency of phage recovery following electroporation of T7 and other T7-like phage genomes is enhanced by ? Red recombination functions....
https://pmc.ncbi.nlm.nih.gov/articles/PMC7697293/
A synthetic cell phage cycle | Nature Communications
...Here, we establish an all-cell-free viral cycle where T7 phages infect synthetic cells, equipped with lipopolysaccharides on the outer leaflet of the lipid membrane, while encapsulating a......
https://www.nature.com/articles/s41467-025-67249-8
Structural changes in bacteriophage T7 upon receptor-induced ... - PNAS
...Bacteriophage T7 provides a tractable model for the study of dsDNA viruses. Here, we report the structure of the mature bacteriophage T7, as well as the structures of the full and empty T7 particles in complex with their cell receptor lipopolysaccharide....
https://www.pnas.org/doi/10.1073/pnas.2102003118
Analysis of engineered T7 bacteriophages containing genetic ... - Frontiers
...We integrated DNA fragments encoding for derivatives of the antimicrobial peptide (AMP) apidaecin into the phage genome to induce the production and release of apidaecin within the T7 infection cycle, thereby also targeting phage-resistant Escherichia coli bacteria....
https://www.frontiersin.org/journals/antibiotics/articles/10.3389/frabi.2024.1515874/full
Editing of Phage Genomes?Recombineering-assisted SpCas9 Modification of ...
...In this manuscript, we summarize current methods of phage genome engineering and their coupling with CRISPR-Cas technologies. We also provide examples of our successful application of these methods for introduction of specific insertions, deletions, and point mutations in the genomes of model Escherichia coli lytic phages T7, T5, and T3....
https://link.springer.com/article/10.1134/S0026893322060073
T7 Phage - an overview | ScienceDirect Topics
...To accomplish T7 genome replication, the phage synthesizes four replication proteins: (1) T7 RNA polymerase (gp1), which synthesizes the RNA primer to the leading strand; (2) T7 helicase/primase (gp4) which performs two activities....
https://www.sciencedirect.com/topics/neuroscience/t7-phage
Homology-Based Enzymatic Assembly of Modular T7 Phage Genome - bioRxiv
...Here, we present a novel, efficient method to design and make synthetic bacteriophages in vitro without using restriction enzymes to allow for modular insertion of DNA fragments into the phage genome....
https://www.biorxiv.org/content/10.1101/2025.04.24.650493v1.full.pdf
T7 Phage as an Emerging Nanobiomaterial with Genetically Tunable Target ...
...It also discusses perspectives and challenges in exploring T7 phage, including the understanding of its interactions with human body, assembly into scaffolds for tissue regeneration, integration with genome editing, and theranostic use in clinics....
https://pmc.ncbi.nlm.nih.gov/articles/PMC8811829/
? Recombineering Used to Engineer the Genome of Phage T7 - MDPI
...This manuscript describes that the presence of ? Red recombination proteins makes in vivo recombineering of T7 possible, so that single base changes and whole gene replacements on the T7 genome can be made....
https://www.mdpi.com/2079-6382/9/11/805
PHEIGES: all-cell-free phage synthesis and selection from ... - Nature
...Here, we describe PHage Engineering by In vitro Gene Expression and Selection (PHEIGES) using T7 phage genome and Escherichia coli TXTL....
https://www.nature.com/articles/s41467-024-46585-1